FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004923964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596291
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA406952.5493472054907285No Hit
GGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCC270441.6941773147878425No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT207191.2979463017707924No Hit
GACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCG182431.1428367384142366No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA159110.9967480866583849No Hit
ACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTT153130.959286245427682No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC152910.9579080505997966No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA149950.9393650656427933No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC135950.8516617584137229No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT133880.8386941979877103No Hit
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT127150.7965339652983072No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC125980.7892044746227348No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT125690.7873877632587042No Hit
GCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCG118560.7427217217913274No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC115860.7258075125400067No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA113760.7126520164556462No Hit
CCGCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTG103230.6466866003754954No Hit
CCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACC102720.6434916941835793No Hit
CGCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGC99320.622192319570805No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG95450.597948619643912No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC92300.5782153755173711TruSeq Adapter, Index 12 (100% over 50bp)
ACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTC90680.5680668499665787No Hit
CTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAA88640.5552872251989143No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG87620.548897412815082No Hit
CGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACC78670.4928299414079263No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT77790.4873171620963847No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG71280.4465351242348669No Hit
ACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGT70470.4414608614594707No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT67520.4229805217219166No Hit
CGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCC64040.40117998535354765No Hit
CCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTC63900.400302952281257No Hit
GACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTC58620.36722627641200756No Hit
GCCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTT57530.36039794749202997No Hit
GGGCCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCC57100.3577042030557085No Hit
TACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTT55330.34661599921317604No Hit
CCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTC54710.3427319956073172No Hit
AGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT54120.3390359276598064No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC53920.33778302327081966No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC50370.3155439703663054TruSeq Adapter, Index 12 (100% over 50bp)
GGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCT49190.30815183447128375No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC48180.30182466730690083No Hit
CCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCA47400.2969383401898526No Hit
ACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCT45550.2853489745917254No Hit
CGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGG45230.2833443275693467No Hit
CCGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG43660.27350902811580097No Hit
CCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAG43600.27313315679910494No Hit
GCCGCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACT42850.2684347653404047No Hit
CCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCA42830.26830947490150603No Hit
CGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTA42330.26517721392903926No Hit
CTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGG41580.26047882247033904No Hit
GCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCC41290.2586621111063083No Hit
ACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAA40580.2542143005254055No Hit
ATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTT38300.23993119049095685No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC36990.23172466674309383No Hit
GGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT36530.2288429866484244No Hit
GACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT36200.2267756944065963No Hit
GTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCAC35450.2220773029478961No Hit
GCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTAC35270.22094968899780804No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT34270.21468516705287444No Hit
GTCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA34260.21462252183342512No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC33490.20979883993582624No Hit
CGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCT33390.20917238774133287No Hit
GTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGC32920.20622806242721411No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT32710.20491251281877804No Hit
GGCCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCG32610.20428606062428467No Hit
CGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCGCCGC31990.20040205701842584No Hit
CCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA31850.19952502394613514No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC31450.19701921516816168No Hit
CACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTT30840.1931978567817522No Hit
CCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGC30380.19031617668708276No Hit
CGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCC30090.188499465323052No Hit
ATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCG28920.1811699746474797No Hit
GGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGG28920.1811699746474797No Hit
TGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT28580.17904003718620226No Hit
CCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACT28300.17728597104162086No Hit
GGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCC28220.17678480928602616No Hit
CCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGC28190.17659687362767817No Hit
AGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACC27530.17246228914402198No Hit
GCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTC27480.1721490630467753No Hit
CACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGG26570.16644834807688572No Hit
GCCCTTTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA25570.16018382613195215No Hit
GCCCCCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA24370.15266639979803182No Hit
ACCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA24230.15178936672574111No Hit
GGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGG23830.14928355794776765No Hit
CGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA23610.14790536311988228No Hit
TCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTAT23550.14752949180318625No Hit
GCCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGT22710.14226729336944205No Hit
GCCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCT22660.14195406727219537No Hit
GGACCTGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCC22470.14076380810265796No Hit
CCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCT22070.13825799932468452No Hit
CCCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGT22030.1380074184468872No Hit
TGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCC21700.1359401262050591No Hit
ACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGA21540.1349378026938697No Hit
TGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAAC20860.13067792777131487No Hit
TCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCG20530.1286106355294868No Hit
TAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTT20170.1263554076293107No Hit
CACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCG20170.1263554076293107No Hit
GTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGC19790.12397488929023592No Hit
GCGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG19760.12378695363188792No Hit
TCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTAC19680.12328579187629322No Hit
GCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACT19620.12290992055959721No Hit
AACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTT19600.12278463012069854No Hit
AGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAAC19550.12247140402345186No Hit
TATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTT18660.11689597949246097No Hit
TCGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG18250.11432752549503818No Hit
CGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCG18100.11338784720329816No Hit
CCACGGGCTGGGCCTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGC18030.11294933066715279No Hit
GGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCGCCGCC17990.11269874978935546No Hit
TCCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTT17650.11056881232807803No Hit
CCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTAC17620.11038087666973002No Hit
GCCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAA16970.10630893740552318No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG16890.10580777564992848No Hit
AACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCG16870.10568248521102981No Hit
GGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCC16490.10330196687195504No Hit
ATGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG16130.10104673897177896No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)