FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004923981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004923981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16738711
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA539360.3222231389262889No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA390560.233327404959677No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG308530.18432124194031427TruSeq Adapter, Index 16 (97% over 39bp)
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC264100.1577779794393965No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG228490.1365039398792416No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA198150.11837829089707086No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT180510.10783984501554511No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)