FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14810190
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC200000.13504215678529444No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC187430.1265547572313387No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT170370.11503566125755309Illumina Single End PCR Primer 1 (100% over 50bp)
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA164110.11080884175017336No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158930.10731124988943423No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)