FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19028163
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT1718780.9032821507782963No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC1665890.8754865091286005No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC769930.40462655275761517No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT769620.4044636363478703No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA632800.3325596906017675No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC632600.33245458324064175No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG626350.3291699782054631No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT588200.30912074907073267No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA529300.2781666312192091No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA526940.27692636435792567No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT524680.2757386511772051No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT491290.2581909772372667No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT486150.25548971805633575No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC472810.24847905706925047No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA458740.24108475421405628No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT448010.23544574428966158No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT439450.23094714923348092No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT435950.22910777041378086No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG417110.21920665699573838No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC414980.2180872635997495No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC403250.2119227168697262No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT392750.20640458041062607No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT384520.20207941250030287No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG362690.19060694403343087No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC350450.18417437353253702No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA346150.1819145652683341No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC329190.17300146104487332No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA301140.15826015364699156No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA300210.15777140441775697No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG296230.1556797679313552No Hit
TTTTAATAATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTT294950.15500708082015063No Hit
GCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCTC285100.1498305432847091No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG284010.14925770816657394No Hit
CTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCT283970.14923668669434878No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA273650.1438131468602618No Hit
TGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCT267900.14079131022789745No Hit
CCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAA264010.13874697205400227No Hit
CAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAA258140.13566207100496247No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG243850.12815215005253003No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA239610.12592387399666485No Hit
CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCT236630.12435777431589166No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT236490.12428419916310365No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA232310.12208745531557619No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC220710.11599122837028461No Hit
CGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGG215010.1129956685782017No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG211410.1111037360779388No Hit
ACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCAT211260.1110249055570945No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA205800.10815547459836244No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG201080.10567494087579553No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA200880.10556983351466981No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG199200.10468693168121379No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC195900.10295266022263946No Hit
GTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCAC193920.10191209734749487No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)