FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21386507
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1945090.9094940094705508No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG1193530.5580761739165727No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA943040.44095092293472704No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC918000.4292426060973865No Hit
GTGTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA811770.37957110060095367No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT806630.3771677160744389No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT796570.37246381561982045No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC752960.35207245390750347No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG690270.3227595792057113No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG680540.3182099816487096No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG654650.3061042179538716No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA642630.3004838518043175No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG631770.2954058837191132No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA622220.29094045137899327No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG590820.27625829687849446No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT583850.2729992326470143No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT563570.2635166182116603No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT535560.2504195752957694No Hit
CGGTGTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG522600.24435967968027694No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT508600.23781349614502265No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG466280.2180253184870255No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC458440.2143594557072831No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT411790.19254663699873945No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA379290.17735013950618492No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG366220.17123880959148682No Hit
GTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT365710.17100034147698828No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT362510.16950407095464445No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT356780.16682481155057252No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA347480.16247627534501075No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT347270.16237808259198194No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC339000.1585111584607996No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC337650.1578799193341858No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT331270.1548967299802628No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG323780.15139452178890175No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA319280.14929039136685576No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTG318740.14903789571621023No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT314720.1471582058725158No Hit
CGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGG310260.14507277883199907No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC302140.1412759923815516No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG298580.1396113914254441No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC296370.13857802959595036No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACC287190.1342856035349765No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA286810.13410792141044817No Hit
TGTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG285850.13365904025374503No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC270010.12625250116814307No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC269990.12624314947737844No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG261590.12231543935622587No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA250470.11711589929108104No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA249560.11669039736128953No Hit
GGTGTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG239280.11188362830825997No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT236720.1106866118903849No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC232380.10865729499445607No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC231180.10809619354857714No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT226130.1057348916305033No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGG219400.10258804768819893No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA217210.10156403754946987No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG215970.10098423272206163No Hit
CCGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACG214940.1005026206476822No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)