FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6477983
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1209501.8670935073463453No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT714991.1037231805023262No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG551410.8512063091243062No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG542680.8377298921593341No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG371650.5737125274950552No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG265280.4095101824132604No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG224990.3473148972450221No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC224500.34655848896176483No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT210280.324607211843563No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC209860.3239588618864853No Hit
GCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGT172800.26674969662624926No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC161920.24995434535718913No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG160760.2481636645233555No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC153120.23636987006603752No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT139230.21492801077125392No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA135030.20844451120047708No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT134360.20741023864990077No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG124960.19289954913435248No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGG122320.188824206547007No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG113990.17596526573163282No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA113420.17508536221845597No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATACCGCTCACACCT111200.17165836958818817No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC110910.17121069937972977No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG106220.16397079152569558No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT104320.16103777981510603No Hit
CGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAA103300.15946321563363164No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT97180.15001583054478532No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT92900.14340883574408886No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA92250.14240543700099242No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC88790.13706426830697147No Hit
GCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCTC86430.13342115902434448No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA85340.13173853651669046No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA84110.12983979735667722No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT78380.12099445151368875No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG74950.11569959353088762No Hit
GCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGT74610.11517473880372948No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT73930.11412502934941324No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG72710.11224172709313994No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC72650.11214910567070029No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG72570.11202561044078072No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG70730.10918522015263084No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC69440.10719385957017795No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC68900.10636026676822091No Hit
CTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCT68240.10534143112138454No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG67310.10390579907356966No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA65980.10185269087615698No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC65280.10077210761436081No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)