FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004924104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13134609
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA347200.2643398063847961No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA274370.2088908775282157No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA231660.17637373141446389No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT197850.15063257688142828No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA193620.1474120775121665No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC188090.143201826563699No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC180820.13766683119383302No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA142530.10851484044938071No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG138100.10514207160639498No Hit
GTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTAC135950.10350517476386241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA45500.032.636954
CCGGTAA10650.032.3587881
CCCGTAT48900.030.7964651
TATCGAA48700.030.6854215
CGGTAAT11550.027.2600542
CGTATCG65150.026.6883183
CCGTATC67000.025.9514032
CTTCGCG21350.025.0923216
CGCTATA1401.8682582E-523.6041851
CGCTAGG5500.023.1750181
GTCCAAG112100.022.9935511
AGTTTAC215050.022.51098494
CGCTAGT1703.3262313E-622.2157041
CGCGTAT1303.0689224E-421.7884791
GAACTCT238450.021.087279
ATCGAAG77300.020.9736256
TTAACGG65100.020.5730084
CGACCGT13950.020.55579232-33
TCAATCG35250.020.3969978
CTTAACG65950.020.165343
Produced by FastQC (version 0.11.5)