FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21513739
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1969660.9155358815127393No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG1216110.5652713366095963No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC942630.4381525684586951No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA935680.43492207467981275No Hit
GTGTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA820700.3814771574573811No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT816540.3795435093825392No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT796520.3702378280223628No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC758950.35277456884644737No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG691150.3212598237805153No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG684170.3180153854241701No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG657410.3055768223273509No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA650970.30258338636533616No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA639100.29706598188255423No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG633440.29443510493457226No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG597880.2779061324486646No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT597270.2776225927069209No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT583760.2713428846561725No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT537800.24997979198316017No Hit
CGGTGTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG533520.2479903655984671No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT508330.23628156872220119No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG486130.22596258139972789No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC458330.21304060628419821No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT409030.19012501732032727No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA391360.18191166119473698No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT375030.17432116286248522No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG370590.17225736539799055No Hit
GTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT369330.17167169314455288No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT356430.16567552483554812No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC343360.15960033725425413No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT343340.15959104086927894No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA342630.15926101920265928No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC340550.15829419516523838No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT326310.1516751690628951No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG324120.15065721490811057No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTG320310.14888625357033475No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA320150.14881188249053315No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT318700.14813789457983106No Hit
CGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGG318150.14788224399301303No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC310180.14417763458039534No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG304000.14130505162305818No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC296610.13787003737472134No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACC295920.1375493120930769No Hit
TGTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG291050.1352856423516154No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA289090.1343745966240457No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC276920.12871774636663574No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC268220.12467381890242323No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG265690.12349782620306028No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA254590.11833833254182363No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA253940.11803620003012957No Hit
GGTGTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG243780.11331363646272738No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT236260.10981819571205173No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC232430.1080379379893007No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC230980.10736395007859861No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT221400.10291098167547724No Hit
CCGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACG218800.10170245162870108No Hit
GACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCA218230.10143750465690786No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA217630.10115861310765183No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGG215870.1003405312298341No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)