FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16224802
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA4100282.527167974068343No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT2654861.636297318142927No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG2261371.3937735572982648No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG1744721.0753413200358315No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG951170.5862444423050587No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT869950.5361852797957103No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG794530.48970089126511374No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG716180.4414106255349064No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC679340.4187046473664208No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC601570.3707718590340887No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT589170.36312923880365383No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC545430.336170512281136No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG521950.3216988410706029No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT488620.3011562174996034No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA474280.29231789700731015No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG413880.25509094040099844No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG405140.24970412581922416No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT373770.2303695293169063No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT368480.2271090889121482No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC316820.1952689468876107No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC294020.18121638710906918No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT279260.17211920367348704No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT274910.16943812318942322No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC271400.1672747685919372No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA263310.1622885752319196No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT262750.16194342464086772No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA259460.15991566491843784No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG258140.1591020956681012No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA257330.1586028599917583No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG255520.15748728397425127No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC241480.14883386558430728No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA241280.14871059751607446No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA239460.1475888580951558No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA228110.14059339522294326No Hit
GCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGT219500.13528670488552033No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA219280.13515111001046423No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT216850.13365340298143547No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC213100.13134212670207007No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG211100.1301094460197419No Hit
GCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCTC198790.12252229642001178No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC197680.12183815864131964No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA188270.11603839603096544No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC182990.11278411902961898No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT180990.11155143834729077No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG178160.10980719518179635No Hit
GTGTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA176320.10867312895405441No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC175660.10826634432888611No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT171310.10558526384482225No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA171200.10551746640729422No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)