FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17542320
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA537100.30617386981881534No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA499570.28477989228334677No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC463630.26429229429174705No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC423830.24160430319364826No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA388520.22147583671943052No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG348420.19861683061305463No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT310050.17674401105441012No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC290690.16570784252025958No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG290510.1656052335152933No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA262330.14954122373779524No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG255090.14541406153804057No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG248010.1413781073427004No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT236230.13466291801768523No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG208610.1189181362556378No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT206470.1176982291965943No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG198980.11342855448994203Illumina Single End PCR Primer 1 (100% over 50bp)
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT196060.11176400840937802No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT187880.10710099918368836No Hit
GACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGG181200.10329306499938436No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT178410.10170262542240707No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)