FastQCFastQC Report
Thu 9 May 2024
EGAF00004924191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17600029
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGCCGGTGTGTAGATCTC949380.5394195657291246Illumina Single End PCR Primer 1 (96% over 33bp)
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG250970.14259635594918624No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGCCGGTGTGTAGATCT237900.13517023182177712Illumina Single End PCR Primer 1 (97% over 34bp)
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC204700.11630662654021763No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA184490.10482369091550929No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT181230.10297142124027182No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG179130.10177824138812498No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA177120.10063619781535586No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)