FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8913681
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT515150.5779318331001524No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC510910.5731751001634454No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA507520.569371957556031No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG491060.550905961297022No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT429220.48152946016353965No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC309110.3467815372796042No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG265150.2974640891905376No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG254880.2859424742707306No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA250220.28071455552425534No Hit
CAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCCAAGGCAA248180.2784259387339529No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA188680.21167461568346457No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG186530.20926259308584189No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC182770.20504435821744127No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACA178360.20009690721487566No Hit
CACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATG176080.19753904139041994No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG167140.18750951486821213No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC162340.18212453418514754No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACAC157520.17671711608257015No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG156260.1753035586532657No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG155290.17421534380689638No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA153470.17217353863123439No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT152290.170849730879981No Hit
AGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCC150350.16867330118724239No Hit
CAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCCAA145670.1634229450212544No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC143980.1615269830724254No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT143370.16084264177728597No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA140260.15735362304305034No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA128220.14384629649636327No Hit
CGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTG127090.14257858229389184No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA124300.13944856227186053No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG122960.13794525516450498No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG121590.1364082919278803No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG117850.13221249447899244No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG117010.13127012285945616No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG113690.12754551122033647No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG112000.12564954927150748No Hit
CACAAGCCCAAGGCAACAAAAGAGCAACTGAAAGCTGTTATGGATGATTT105910.11881735502986925No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC105550.1184134814786394No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT102280.11474496338830165No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT98440.11043697884184996No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT94600.10612899429539827No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT94000.10545587171001519No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT93580.10498468590024704No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA90610.10165272910260081No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC90590.10163029168308804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)