FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10351653
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA641760.6199589572795765No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT531120.5130774766117063No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC504020.48689808284725156No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC428410.41385660821513237No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG381800.36882998299885056No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG307760.29730517435234743No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA289700.2798586853713122No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT272080.26283724927796553No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA268830.2596976540848114No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG261320.2524427741153997No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG259770.2509454287155878No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC223340.21575298167355494No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT202440.19556296950834806No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG198160.19142836414628658No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT189600.1831591534221636No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA187130.18077306107536642No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC184410.17814546140601892No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG176380.1703882462057026No Hit
CAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCCAAGGCAA169340.16358740000268554No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA164510.15892147853101335No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC160110.1546709496541277No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC158270.15289345576015734No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT154000.14876851069099786No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA152620.14743539027052008No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG150870.14574483901266783No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG145650.14070216611781713No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC140910.13612318728226305No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT134340.12977637484564059No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA134000.12944792488697215No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA127660.123323299187096No Hit
GTGAAACACAAGCCCAAGGCAACAAAAGAGCAACTGAAAGCTGTTATGGA125330.12107245094092703No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT123560.11936257909727074No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA121590.11745950139557422No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT121560.11743052051686818No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC121340.11721799407302388No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA119630.1155660839867797No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT117850.1138465518502214No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG115770.11183721092660273No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG114730.11083254046479339No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG113010.1091709700856472No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG111950.10814697903803383No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACAC109850.10611831752861114No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG108380.10469825447201524No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT105080.10151035781435101No Hit
AGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCC103560.10004199329324505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)