FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17931778
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA568680.3171353114007992No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA515590.2875286544368328No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC492250.27451265568868854No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC453780.2530591221907833No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA412310.22993258114170273No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG373080.2080552190641664No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT325800.1816886200576429No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC302010.16842167017682239No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG300550.1676074731685837No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA279050.15561758571849374No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG270720.1509722014180635No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG260220.14511667498894978No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT256030.14278004110914153No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT223420.12459444902786551No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG222760.12422638736660693No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG213880.11927428501512789Illumina Single End PCR Primer 1 (100% over 50bp)
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT198100.11047426529594555No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT196840.1097716021244519No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT190530.10625270957514642No Hit
GACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGG183990.10260555311358416No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)