FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16645791
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1274390.7655929357757766Illumina Single End PCR Primer 1 (100% over 50bp)
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC462160.277643759915044No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACA318340.19124354018382184No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG307920.18498369948295038Illumina Single End PCR Primer 1 (100% over 50bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT263620.1583703652172492No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACAC252510.15169600531449662No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA225430.13542762852182874No Hit
AGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCC190850.11465360823045298No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)