FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004924315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8428319
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA230090.2729963116014No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA181550.21540475627464978No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA158710.18830563959432478No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT129920.154146989453057No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC123700.14676710741489496No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA123620.1466721893179411No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC117140.13898382346467902No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG97710.115930590667012No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA89380.10604724382169209No Hit
GTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGA86950.1031641066267188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT853.9885526E-738.669951
GTATCGA29200.032.950084
CCCGTAT31350.030.7051221
TATCGAA31850.029.9152645
CTTCGCG14250.029.498496
CGTATCG43550.024.773993
CCGTATC44000.024.0960182
GTCCAAG71350.023.9553171
AGTTTAC124550.022.46227894
TCAATCG21900.021.3269148
ATCGAAG48450.021.3044646
GTCGGTA7100.021.1634351
CGGGTAA3850.020.733981
CTAATCG2058.0421523E-720.5050457
CTTGTTA88600.020.2427569
GTCGTAT2109.627947E-720.1241591
GAACTCT148200.019.6341449
TAGGTAT36250.019.40715694
GTCGTTT6050.019.4034581
TCGACCG9000.018.9515230-31
Produced by FastQC (version 0.11.5)