FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17923090
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT2058951.1487695481080549Illumina Single End PCR Primer 1 (100% over 50bp)
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC1436790.8016419043814431No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT941460.5252777283381381No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG618430.34504652936519314No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT561370.3132105010910507No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC557290.31093410790215303No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC530920.29622124310038056No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT516290.2880585881117598No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA504000.2812015115697126No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG456900.25492256078611447No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT434440.24239123945703558No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT414880.23147794269849672No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT406090.22657365443123925No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG372760.20797753066017075No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC362010.20197968095903104No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC359830.20076337283359064No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT353490.19722603635868594No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT321880.17958956853979977No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA319410.17821145795730536No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT290830.16226554684487998No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT290200.16191404495541784No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC278900.15560932852538262No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG273110.1523788587793734No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT272030.15177628411172403No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC270860.1511234948884372No Hit
CCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAA253890.1416552614532427No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA245590.13702436354445577No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG244340.13632693916060232No Hit
CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCT243870.13606470759227343No Hit
CTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCT237890.1327282293399185No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA229220.12789089381351096No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA221490.12357802142376119No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG218670.1220046320137878No Hit
TAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTT215520.1202471225664771No Hit
GCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCTC210850.11764154506840058No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA207600.11582824167038161No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT199680.11140936077428612No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC194740.10865313960929729No Hit
CATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAA189580.10577417175275024No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC188980.10543940804850058No Hit
CCCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTAC186470.10403897988572283No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC184640.10301795058776136No Hit
CAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAA183650.10246559047574944No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA180360.10062996949744714No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG179440.100116665150931No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)