FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004924450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3552207
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT206540.5814413405525072No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC147530.4153192648964433No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT125960.35459645229008335No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC116440.3277962123265902No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA110090.30992000184673923No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT96080.27047973274080034No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT93900.2643427030012609No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG87790.24714212882301062No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC87250.2456219471444091No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG87040.24503076538050853No Hit
CTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACTATTCCTC83610.2353747965701323No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA76030.2140359500445779No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT68060.19159919452892243No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT67010.18864328570941952No Hit
CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCT57570.16206825784645995No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA56350.15863377331332323No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA55960.15753586432322217No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA55750.15694468255932156No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC54660.1538761676895519No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT49820.14025083560727175No Hit
CCAAAATGAACGAAAATCTGTTCGCTTCATTCATTGCCCCCACAATCCTA49250.13864619939097017No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC46770.13166462427442996No Hit
CCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAG44340.1248238067207232No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA43660.12290950386618799No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT41980.11818004975498331No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT41710.11741995891568256No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG41340.11637835295071487No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC41030.11550565606114732No Hit
CAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCAC39740.11187411094004376No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA39540.11131108068870986No Hit
CTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACG36310.10221814212966755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)