FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15539268
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT219860.14148671610528887Illumina Single End PCR Primer 1 (100% over 50bp)
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA204540.1316278218510679No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT178210.11468365176532125No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA161550.10396242602933421No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA156130.10047448824487744No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)