FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004924530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12368107
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTC153870.12440869083684351No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA153340.12398016931774603No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC124350.10054085075428276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT16800.023.3026581
GTATCGA14500.021.7144154
TCGACCG19400.020.72303230-31
CGTATCG21350.020.251683
CGATCCG20100.019.87335682-83
CCCGTCG19400.019.4502371
GTTCGAC21150.019.3418428-29
CCGTATC26550.018.5864282
ACGACTT22200.017.421981
CGCTAGT2454.0291525E-617.3265881
CGACCGT21150.016.89150432-33
CGGGTGA27750.016.6571141
CGCCCGA81800.016.6029749
AAGTTCG24850.016.2727526-27
TCGCCCG86600.016.1168388
CGCCCTT21450.014.5128691
GATCCGA24000.014.39187584-85
CTCCGAT30900.014.1958411
GACCGTC30200.013.85313832-33
GTCCGTT8950.013.7020951
Produced by FastQC (version 0.11.5)