FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16642808
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTAC1022280.6142473073053538No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA660350.39677799563631333No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC596370.35833496366718887No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT504120.30290561544662414No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG501640.3014154822912095No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG482360.2898308987281473No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAA441280.2651475640408758No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA386290.2321062647601294No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTA382650.22991913383847246No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT345170.2073988956671254No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT344550.20702636237827174No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT340730.20473107663081855No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACCC319350.19188468676680043No Hit
CTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAG311910.18741428730055648No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG309120.18573788750071504No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT286060.17188205259593212No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC284480.1709326935694986No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT256330.15401848053525583No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACC244160.14670601259114446No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG222300.1335712098583364No Hit
CCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA217890.13092141662632895No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG217170.13048879732314403No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG214770.1290467329791944No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA209960.12615659568986196No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC205930.12373512931231317No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC202760.121830402658013No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT197540.11869391270992252No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT193550.11629648073810622No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG192000.11536514751597207No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT187970.11294368113842328No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT187290.11253509624097088No Hit
GCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCTC183950.11052822336230761No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA176320.10594366046883434No Hit
CCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAAT173300.10412906283603104No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC170280.10231446520322773No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG166760.10019943749876824No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)