FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18324551
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA2191461.1959147048132313No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC1517910.8283477177694558No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT1454710.793858468892362No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC1387250.7570444700118437No Hit
CCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTA1235870.6744339874957919No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG1088390.5939517972363961No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG821480.4482947494866314No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGG786390.4291455763363588No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTG784570.4281523732832526No Hit
CGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGG771330.4209270939298867No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA612410.3342019130509664No Hit
CCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCC582260.31774857675912493No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG558230.30463502216234384No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG518410.2829046125059217No Hit
TTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC503420.2747243302168768No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC493010.2690434270394947No Hit
CCGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACG459960.2510075144542423No Hit
CCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAG455030.24831713475544367No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT432790.23618041173287138No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG385600.21042807542733244No Hit
AAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCG380180.20747029490654367No Hit
CTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCC374490.20436517107567873No Hit
CCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGC347960.18988732657078475No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA339690.1853742555547473No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT335380.18302221975316066No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG330200.18019541106355078No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG325250.17749411704548723No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC301130.16433144801201405No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC290760.15867237347316177No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT289480.15797385704020797No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTC284410.155207077106555No Hit
CGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGC278740.152112867594955No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT276050.1506448916538255No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG266490.1454278470452018No Hit
GTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTA264790.14450012990768504No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT259710.14172789281439965No Hit
GGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCT258340.1409802619447538No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT244270.1333020383418944No Hit
CTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATA240060.13100457413663233No Hit
GTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAA237830.12978762753859563No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC229800.12540552835373703No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG217580.1187368792828812No Hit
GGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTC211600.11547349782267517No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC209970.11458398080258556No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA200080.10918684992609094No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA197730.10790441741246484No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG195880.1068948428804613No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA191660.10459192151556673No Hit
CTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG189630.10348411811017907No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTG189370.10334223195973534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)