FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004924553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15032208
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA228420.1519537249617621No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG191090.12712038045242588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT24900.030.0582521
GTATCGA20800.028.6971284
CCCGTCG26000.028.4244631
ATCGCGT9950.019.8388194
CCGTCGG34500.019.2087042
CGCGTAT2251.8213632E-618.8289431
CGTATCG54350.018.246563
CCGTATC59600.016.9547042
CCCGTCT37100.016.8749961
TAAGCCG74400.016.45599666-67
TATCGAA41150.016.3329355
CGACGCA8400.016.2261984
GACGAAA76350.016.09742246-47
TCGACGA79000.015.64668644-45
TCGACCG9500.015.58390630-31
AGTTTAC186800.015.47346794
GTCCGTT11350.015.3451751
CTTACTT92600.015.0977211
ATTCGCA36100.014.84181692-93
TGAACTC205200.014.7732528
Produced by FastQC (version 0.11.5)