FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20196401
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC2924311.4479361941763782No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC2727251.350364354520392No Hit
CCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTA2226641.1024934591068973No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA2146111.0626200182893972No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT1385920.6862212727901372No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG1052050.5209096412771761No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC1022860.5064565711484933No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG932740.4618347595692916No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT883340.4373749560627163No Hit
TTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC848240.41999562199225493No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG815340.40370559091196495No Hit
AAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCG786340.38934659695061513No Hit
CCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAG758130.37537876179028135No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT744660.36870925666409576No Hit
CTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCC689040.34116969652167234No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG668220.3308609291328688No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC655090.32435977083243694No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT564810.27965873721758644No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA552450.27353883496371456No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC541720.2682260071980151No Hit
CCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGC531340.2630864776352975No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG511460.25324313970593076No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG505640.2503614381592047No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA498320.24673703002827088No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT483340.23931986694064947No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT463150.22932303631721315No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG461240.2283773232666553No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT442300.2189994147967254No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTC438150.21694459324708396No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT423520.20970072836244438No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA423170.2095274301594626No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG419060.20749241411873331No Hit
CGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGC418190.20706164429989282No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT409390.20270443233920735No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTG397630.19688161271901858No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG388850.19253430351278922No Hit
CGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGG380750.1885236879580674No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA360010.1782545315870882No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT353580.175070796029451No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC348950.17277830837286307No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC333060.16491056995748896No Hit
GGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTC324530.1606870451819609No Hit
AGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGG316530.15672594339951956No Hit
CCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCC312610.1547850035261233No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA310100.1535422078418823No Hit
CCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCC306880.15194786437444968No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA305940.15148243491501281No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC299360.14822442869895483No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG297790.1474470624741507No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG293290.14521894272152747No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT290730.14395139015114625No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG290500.14383750847490107No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT290120.14364935614023508No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT284890.14105978584996404No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC277820.13755916214973152No Hit
GCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGG269400.13339010252371203No Hit
GCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCTC264350.13088965702354594No Hit
GTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCT261960.12970627786604158No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC258200.12784456002829414No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC257250.12737417919162924No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC254290.12590857153212595No Hit
CCGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACG252650.12509654566672548No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA247100.1223485313051568No Hit
CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCT245110.12136320723677452No Hit
CTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG245030.1213235962189501No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGG244980.12129883933280983No Hit
CAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCAC241270.11946187838120267No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG233930.11582756749581274No Hit
CCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAG227780.11278247050056096No Hit
GCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGT222720.11027707362316681No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG214130.10602384058427043No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC211940.10493948897132711No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)