FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18811672
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA2705721.4383197835896777No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT1541920.8196613251602515No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA1334030.7091501489075506No Hit
ACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTT1030310.5476971956559735No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC1019260.5418231829685314No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC991760.5272045993572502No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC979680.5207830542654582No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC922610.49044550638560996No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT836170.4444953112089133No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG687920.365687855922642No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA677420.36010621490742556No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG666760.35443952031483433No Hit
CGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGG592100.314751394772352No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG590830.31407628200194004No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC590670.31399122842456534No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT548060.29134039759995817No Hit
ACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTC535360.2845892698958391No Hit
CGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCC529830.2816496056278251No Hit
CACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGG526330.27978905862275294No Hit
GCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCC511870.27210234156751195No Hit
AGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACC493590.2623849703524493No Hit
ATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTT418580.2225107901094597No Hit
GCCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTT418320.22237257804622576No Hit
GCACACCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACT416510.22141040945217416No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC409430.21764678865334244No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC406610.2161477193521129No Hit
TACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTT396640.21084781831195018No Hit
CCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTC394840.20989096556648448No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT393690.20927964297910362No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT385150.20473990828672753No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG379830.20191187683901782No Hit
CGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCGCCGC370570.19698940104845544No Hit
GGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGG361460.19214666298668187No Hit
GTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGC359320.1910090713892949No Hit
AGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT349800.1859483835354986No Hit
CCACGGGCTGGGCCTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGC349390.18573043374347586No Hit
CTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGG346550.18422073274507444No Hit
CCGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG331920.17644364626387277No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT328210.17447146643849626No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA318050.16907056427520106No Hit
GTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGC316060.16801271040660287No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT300120.15953924776064562No Hit
ACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGG274040.14567551464856499No Hit
GTCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA272030.144607029082795No Hit
CGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA260620.13854164584625972No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG256310.13625051510572797No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT252660.13431023037186698No Hit
GACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT251450.13366701269297063No Hit
GTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCAC249780.1327792659791219No Hit
GGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCC244070.1297439164365613No Hit
GGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT242330.12881895878261113No Hit
GGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTC240060.1276122611536072No Hit
CCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGG234500.1246566493398354No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC233890.12433238257609425TruSeq Adapter, Index 2 (100% over 50bp)
GGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAG230210.1223761502964755No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC227220.12078671156928529No Hit
CACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTT226640.1204783923513019No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC223000.11854342346602684No Hit
GGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCGCCGCCG220550.11724104056247632No Hit
CCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA218540.1161725549967063No Hit
GGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACC209690.11146802899816667No Hit
CCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCC206720.10988922196814828No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG206490.10976695745067212No Hit
GCCCTTTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA198030.10526974954698338No Hit
GGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCGCCGCCGG197240.10484979750869568No Hit
CAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTCTTCCGT195190.10376004854858195No Hit
CCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGC194270.10327099047867727No Hit
TCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTAT193310.1027606690144289No Hit
TGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT188500.10020374584460115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)