FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18148345
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA7300524.022691876311586No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT4362632.4038720886119367No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG3778822.082184353449309No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG3262511.7976900924023649No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG2089181.1511683296741384No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG1612820.8886870951593658No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC1450990.799516429735053No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC1446980.7973068618653658No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG1316270.7252837655444615No Hit
CCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTA1125540.6201887830543226No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC1039220.5726252173407548No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT952920.5250726719158139No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC939270.5175513249279755No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGG801580.44168214787629395No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG709150.39075188398721755No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA699210.38527480053966356No Hit
CGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGG673190.3709374050361066No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA665310.3665954113171201No Hit
CCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCC635660.3502578334277864No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT631400.34791051195026323No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTG623270.34343076462344085No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC558210.3075817657202351No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA553080.30475506168744315No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG535360.29499108596403695No Hit
TTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC501830.27651557208109057No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG500080.2755512968262395No Hit
GCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGT473140.2607069680458466No Hit
CCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAG424730.2340323594245095No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT422370.23273196536653892No Hit
CCGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACG413940.22808691371031353No Hit
AAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCG404230.2227365635819685No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG383690.21141872716217372No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC370600.2042059482558878No Hit
CCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGC369940.2038422787312011No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT369100.2033794266088726No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT365150.2012029196050659No Hit
CTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCC356820.19661296939197487No Hit
CGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGC318550.1755256471044605No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTC309280.1704177433259066No Hit
CTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATA289460.15949663729667912No Hit
CGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGG288250.15882990983475354No Hit
GGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCT281040.15485709578476717No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG276140.15215712507118417No Hit
GCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAAC270920.1492808297395713No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG269860.14869675444234723No Hit
CGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAA264380.14567719535858503No Hit
GGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTC263780.14534658669977896No Hit
GACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCC262910.14486720414451015No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC255640.14086132922864317No Hit
GTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTA246210.1356652631410743No Hit
GCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGT235020.12949941165434092No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA229830.12663964675566836No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC229370.12638618011725036No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT224260.12357049637308525No Hit
CCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACC222740.1227329544374432No Hit
AGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAA217500.11984563881720343No Hit
GTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAA213610.11770219267927737No Hit
TGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACC210300.1158783349115305No Hit
CTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG203790.11229123096348455No Hit
CGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAG199670.11002105150634948No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACC198440.10934330375579701No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA197740.10895759365385659No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG194900.10739271266884116No Hit
CGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAA193730.10674802578416931No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT192700.10618048091988552No Hit
AGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGG190110.10475335354270597No Hit
GCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGG190100.10474784339839253No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTG188740.10399846377176541No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG188730.10399295362745198No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA184890.10187705821109308No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)