FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18453973
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA851480.4614074161699489No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA338490.18342391635665664No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT309380.16764953541440641No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC264350.14324828588402075No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC255560.13848508394371228No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC227480.12326884839378491TruSeq Adapter, Index 8 (100% over 50bp)
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT223030.12085744354345809No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC222960.12081951133232935No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC213970.11594793164593879No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)