FastQCFastQC Report
Wed 1 May 2024
EGAF00004924639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18987153
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT1779500.9372126511015106No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC1694790.8925982742120422No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC790760.41647107388874993No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT790090.416118203713848No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC645760.3401036479771349No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG644320.3393452404370471No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA643670.3390029037002019No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT599710.3158504068514116No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA544830.28694665282362236No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT543540.2862672460689604No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA540000.28440282753291135No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT505960.26647491596028117No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT499210.26291988061611976No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC484140.25498293504034014No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA472230.24871027267753099No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT462670.24367528928639276No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT455650.23997805252846488No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT450360.23719195816244804No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG434070.22861247286520525No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC424170.22339842102710186No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC415360.2187584415630927No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT407950.21485580276305777No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT395660.2083830050771698No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG372180.19601674879851655No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA355130.1870369928551163No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC353740.18630491891017045No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC340270.17921064837893286No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA308650.16255728281117238No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA305640.1609720003836278No Hit
TTTTAATAATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTT301630.15886004605324452No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG301390.15873364479656324No Hit
GCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCTC296250.1560265512159722No Hit
CTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCT291450.15349852608234632No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG289780.15261898400460563No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA277550.14617778663288805No Hit
CCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAA273860.14423436731141315No Hit
TGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCT272780.14366556165634733No Hit
CAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAA264740.13943111955752396No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG247860.1305408978376063No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA245850.12948228731290046No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT241380.12712806390721137No Hit
CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCT236900.12476857378249388No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA235370.12396276577115062No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC224340.11815357468283949No Hit
CGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGG222920.11740570058080851No Hit
ACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCAT214730.11309225769655935No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG211570.11142797448358897No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA211480.1113805740123335No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG207240.1091474851442973No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA206050.10852074557991921No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG204930.10793087304873986No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC202980.10690386283820434No Hit
GTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCAC200810.1057609848090443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)