FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16465972
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1800191.0932789148432902TruSeq Adapter, Index 13 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA312030.18949989712116602No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA301380.18303201292945234No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT249700.1516460734902258No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC184620.11212213891776325No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)