FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14533880
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC595850.40997311110316037No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA503460.34640440130233635No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT383070.26357036111485715No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACA333360.22936751920340612No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACAC264910.18227066688317228No Hit
AGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCC243330.16742260153517163No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA222810.1533038665518086No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC178730.12297473214310287No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG174230.11987851833096186No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC158860.1093032280437158No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT157320.10824363487244976No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC153820.10583546857411785No Hit
AGGAGGTCCCCCACGGCCCTTCAGGATGAAAGCTGCGGTGCTGACCTTGG148470.10215441437523909No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)