FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004924788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13126935
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA297800.2268617921853045No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA234190.1784041743179196No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA195390.14884662718296388No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA165560.12612235834183685No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT164680.12545198098413682No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC159090.12119356117783779No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC149390.11380417439409884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA42500.032.466314
TATCGAA46500.030.624585
CCCGTAT47400.030.4477961
CGCTAGT1352.3095708E-829.6046261
CTTCGCG19900.027.7794866
CGTATCG62500.025.2612863
CCGTATC64950.024.4445762
GTCGGTA10400.023.4844381
TAGGTAT55050.022.76896794
AGTTTAC191350.021.9908594
ATCGAAG72050.021.8515996
CGCTAGG4950.021.5306381
CCGGTAA9600.021.2783241
GTCCAAG113900.020.1566181
GTCGTTT9550.019.9947471
TCAATCG32850.019.9247828
GAACTCT231850.019.6661249
GTCCCGT10400.019.6415311
CTTGTTA133550.018.67689
CGGTAAT10500.018.5323542
Produced by FastQC (version 0.11.5)