FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21487690
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT4356622.027495742911406No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG2650031.2332782165044265No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA759110.3532766900490467No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC725480.3376258685787072No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG721220.3356433381159166No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT487930.22707419922755773No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT474870.22099630067261766No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA453050.21084164933503788No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC439470.20452175175647078No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC427290.19885339001074567No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG397570.18502221504498623No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT380300.1769850551641428No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA363360.169101471586755No Hit
CACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATG345720.16089212009294623No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA329910.15353441900920944No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT321190.1494762815360795No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG317590.14780090368020016No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATG310090.1443105331471182No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT299160.1392238998235734No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT294840.1372134463965182No Hit
CGTGCCTCCTGGTCTCAGTATGGCGCTGTCCTGGGTTCTTACAGTCCTGA291500.13565906805245237No Hit
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAG263400.1225818131218386No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT257100.11964990187404974No Hit
GACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGG246020.11449346113984332No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG244610.11383727147962393No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG236620.11011886340504726No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT235140.10943009695318574No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGA233140.10849933147769723No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG229840.10696356844314117No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG228850.10650283953277434No Hit
CAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTC227210.10573961184287374No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA223120.10383619644549973No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC222950.10375708138008322No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT222180.10339873667202013No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA219400.10210497266109107No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT218580.10172335881614078No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)