FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16556693
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1189750.718591568980593TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC294140.177656250556799TruSeq Adapter, Index 1 (100% over 50bp)
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT266990.16125804833127003No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA222760.13454377634470846No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)