FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19282003
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC1525860.7913389495894176No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT1302520.6755107340248833No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC688980.3573176500387434No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA663780.3442484683774813No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG603560.31301727315362415No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC593370.3077325524739312No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT573370.29736018607610426No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT544980.2826366119743887No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT504180.26147698452282164No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT473230.2454257475221843No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT449190.2329581631119962No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT438340.227331154341175No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG432970.22454617396335846No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA423320.2195415071764069No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA407820.211502923218091No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT400690.20780517459726566No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC381550.19787881995454518No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA371360.1925940992748523No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA369730.1917487514134294No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC365830.18972613996585314No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT365290.1894460860731118No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC362830.18817028500617908No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG350610.18183276913710675No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT350020.18152678432837085No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT320540.16623791625797382No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC313670.16267500840032023No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT305070.1582148908492546No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG268940.13947721095158008No Hit
TTTTAATAATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTT266810.1383725539302115No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC258010.13380871271516762No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA240700.12483142959784832No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA239570.12424539089637109No Hit
CTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCT238460.12366972456129169No Hit
CCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAA231040.11982157662769786No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA229320.11892955311748474No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA219470.11382116266655491No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA218940.1135462949570125No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA215150.11158073152462429No Hit
CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCT209440.10861942091804466No Hit
CGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGG208680.10822527099492725No Hit
CCCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTAC207680.10770665267503589No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG206190.10693391137839778No Hit
CAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAA204740.10618191481455531No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA202200.10486462428203128No Hit
TGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCT199330.1033761897039431No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG197010.10217299520179518No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG194710.10098017306604506No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)