FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18198081
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC559440.3074170293010565No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA463440.2546642143201802No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA454760.24989448063232603No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT429590.23606335195452752No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT364870.20049916252158675No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG343460.18873418576387258No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT335860.1845579212445532No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA299390.16451734663671405No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC275750.15152696594767326No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC272440.1497080928478118No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG269770.14824090518115618No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT262280.14412508659566908No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA245370.13483289804018345No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG241540.13272828052584226No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT235440.1293762787405991No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT231580.12725517597157635No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC225440.1238811938467578No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA221610.12177657633241658No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT218310.11996319831744896No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG213840.11750689536990191No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA212540.11679253433370254No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC196640.10805534935249492No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT194190.10670905355350381No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC190330.10458795078448106No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA186530.10249981852482137No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC186190.10231298563843079No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA183990.1011040669617857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)