FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6427229
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1231911.9167046949781936No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT722791.124574836216354No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG561200.873160113012933No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG551040.8573523675599546No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG376090.5851510814380506No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG262210.4079674148843926No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG231620.3603730316750811No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC223130.34716360658691325No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT210860.32807295336761766No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC207870.32342087079828646No Hit
GCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGT172480.26835826139071756No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG165640.2577160390582007No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC164230.2555222476124625No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC150840.2346890082802402No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT138180.21499156168233619No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT136720.21271997621369956No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA136510.21239324131752582No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG125110.19465620409666437No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGG124070.19303808842037526No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG115440.17961084006809155No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATACCGCTCACACCT112860.17559666848652816No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC111890.17408746444229697No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA111110.17287387768508014No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG108330.16854853001192271No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT107600.1674127372776044No Hit
CGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAA103920.16168709719227367No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT98770.15367431283372662No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA93650.1457082048889187No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT92830.14443238291338306No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC90840.14133618080202215No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA86350.1343502775457355No Hit
GCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCTC86000.1338057193854459No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA82920.12901360757489735No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT78800.12260338008805971No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG74930.11658212271571466No Hit
GCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGT74720.1162553878195409No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG74440.1158197412913092No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG74140.11535297715391812No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC73820.11485509540736763No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT73150.11381265550052752No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG71790.11169665807768792No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC70990.11045195371131168No Hit
CTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCT68260.10620440006105275No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC68140.10601769440609632No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG67230.10460184318934335No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC67030.10429066709774928No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA66220.10303040392679334No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT65070.10124114140012748No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA64290.10002755464291065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)