FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16465972
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1863321.131618588929946Illumina Single End PCR Primer 1 (100% over 50bp)
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA191680.11640976918945325No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG189510.11509189982832474No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC185960.11293593843108685No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG180920.10987508056007869No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA173840.10557530402699579No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA167970.10201037630818272No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)