FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14810190
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT231010.15598044319485435No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA213160.14392793070176682No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA200950.1356836070300246No Hit
GTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTAC183360.12380664934075794No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG165940.11204447748475879TruSeq Adapter, Index 15 (97% over 39bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA163330.11028217733871071No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)