FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004924999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004924999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16185083
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA4116642.543477843147298No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT2650541.6376437488766664No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG2264701.3992513971043583No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG1754921.0842823604920655No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG950100.5870220128003051No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT864260.5339855223479546No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG793200.4900808973299674No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG718670.44403232284937927No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC684080.4226607920391882No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC607730.375487725333259No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT583110.36027618764760116No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC539900.3335787650888167No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG515160.31829308505863085No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT485560.3000046400750617No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA473300.2924297638757861No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG409440.25297367952947786No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG406970.25144758293794356No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT369740.2284449205481368No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT366520.22645543430330262No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC315980.19522915019960047No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC290120.17925147495382013No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT279200.17250452160177368No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT274020.1693040437296491No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC269090.1662580290752911No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA266280.16452186250759418No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT260890.16119163553254562No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG256920.1587387596344115No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA256480.15846690437114225No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA254590.15729916244482653No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG246730.15244283887824364No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC240030.14830322464209791No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA239630.1480560834936713No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA238110.14711694712965018No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA233790.1444478227266428No Hit
GCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGT218370.134920531454797No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC212640.1313802345035858No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA212620.1313678774461645No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT212450.13126284245808315No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG209120.12920539239743165No Hit
GCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCTC199600.12332343306487832No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC198020.12234722552859321No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA187550.11587830596852669No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC182750.11291261218740738No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT180770.11168926350269566No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG179130.11067598479414656No Hit
GTGTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA177280.1095329569826735No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC176380.10897688939871361No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA173340.10709861667067139No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT172860.10680204729255946No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)