FastQCFastQC Report
Wed 1 May 2024
EGAF00004925067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16176736
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA580900.35909592639701854No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA516430.3192423984665386No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA410100.25351220419248977No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG349330.21594591146199085No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG321000.19843310789024438No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC273460.16904522642886674No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG177550.10975638101530494No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC173010.10694988160776069No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC166900.10317285266941366TruSeq Adapter, Index 3 (100% over 50bp)
GTGGGGATCAATAGAGGGGGAAATAGAATGATCAGTACTGCGGCGGGTAG163040.10078671000132534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)