FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20432685
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC2965041.4511259778144674No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC2760521.351031447898306No Hit
CCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTA2248561.1004721112276727No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA2156771.0555489892786973No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT1400560.6854507863259283No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG1055730.5166868671444795No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC1030750.5044613568897088No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG938970.45954312906013084No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT900340.4406371458278734No Hit
TTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC855050.41847167907692995No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG822730.40265388518444833No Hit
AAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCG797500.39030602194474195No Hit
CCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAG767570.37565792258824526No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT748360.3662563192257895No Hit
CTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCC688260.3368426616472578No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG678290.33196322460802385No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC663490.324719927899833No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT566600.2773008050581703No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA550880.26960724936541625No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC539360.2639692238195812No Hit
CCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGC534310.26149769352388097No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG513590.2513570781324138No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG505120.24721175900279382No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA500080.24474512282649097No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT481830.23581335492618813No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG467090.22859942293438187No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT466780.22844770523306165No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTC445910.2182336780506331No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT442130.21638370091840597No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA439770.2152286887406134No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT431750.21130360498387754No Hit
CGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGC427490.20921871012057397No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG421670.2063703326312719No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT418440.20478953206590325No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTG397190.1943895283463725No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG392160.19192778628946708No Hit
CGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGG384020.18794397309996214No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA360490.17642811015781823No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT357980.17519968618906423No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC349300.17095159055209827No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC337640.16524504733469927No Hit
GGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTC327750.16040476325064473No Hit
AGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGG321680.15743403277640702No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA316970.15512890254021927No Hit
CCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCC315020.15417454925772114No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA311530.1524665015880194No Hit
CCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCC310560.15199177200646904No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC299580.14661802890809505No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG299570.1466131347886976No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG297490.14559515795403297No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT295490.14461633407454771No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG290440.14214480377884747No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT288610.14124917992911848No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT284590.13928174393135312No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC278570.13633548405410253No Hit
GCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGG268620.13146583525366343No Hit
GCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCTC265420.12989971704648703No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC264990.1296892699123977No Hit
GTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCT263630.12902366967434775No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC258420.12647383346828867No Hit
CCGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACG257380.12596484505095634No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC253190.12391420902343475No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA253010.12382611487428109No Hit
CTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG249160.12194187890627198No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGG243830.1193333132674438No Hit
CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCT242800.11882921896950889No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG241680.11828107759699717No Hit
CAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCAC241160.11802658338833101No Hit
CCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAG226530.11086648670989643No Hit
GCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGT222930.10910460372682297No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC214200.10483203749286987No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG208550.10206686003332406No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)