FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004925233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13134609
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC522260.39762127673537906No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG335860.25570612722464753No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACAC287030.21852953521494245No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA280080.2132381710030348No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA256870.19556729857736913No Hit
AGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCC239120.1820533827843676No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC228870.17424957225601462No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG216280.1646642088850913No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT206690.15736288762002737No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC194190.1478460455122798No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA172080.13101265519209596No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG161920.12327736592691871No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG151680.11548116887225193No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT137720.10485275960631946No Hit
CAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCCAAGGCAA137290.10452538023781294No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC135890.10345949392174521No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA132530.10090136676318268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)