FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20069929
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG8916474.442701316980244TruSeq Adapter, Index 13 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA539170.26864569376403874No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA430650.21457475011496052No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAAACTCGTATG320360.1596218900425607TruSeq Adapter, Index 13 (97% over 39bp)
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC282820.1409172897422806No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT218050.10864512774310263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)