FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16696036
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA2779341.6646705840835514No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT1562070.9355933348490624No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG1525490.9136839427035255No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG1109880.6647565925229199No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT897820.5377444083134464No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG878700.5262925882526847No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG639470.3830070802434781No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC600050.3593966855366148No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG587330.35177811068447623No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT509330.3050604347043813No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT416100.24922083301689096No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC404840.24247671722796957No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC399100.23903877543148566No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT397370.2380026013360297No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG362390.2170515204926487No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG357280.2139909137713886No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG327070.19589679849755953No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC321300.1924408883641602No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC313530.18778708910306613No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG311480.18655925274717902No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG303540.1818036329102309No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA293630.17586809228250347No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC290560.17402933247149202No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA279720.16753677339938652No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC271390.16254756518253793No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC269210.16124186603335067No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG259410.15537220930764642No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC247320.14813096953073174No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT244440.1464060091868513No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT243940.14610653690492761No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT235560.14108738145988664No Hit
CGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTG232540.13927856887706758No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT231240.138499940944066No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT230110.13782313358691847No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG219960.13174384626386768No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG219480.1314563528732209No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC218850.13107901779799708No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC217860.13048606267978818No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT215240.12891682792250808No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA212250.12712598367660444No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC203500.12188521874293994No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA195870.1173152717207845No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA195740.11723740892748435No Hit
CCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCA194430.11645279154884429No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG194240.11633899208171329No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT192420.11524891297551107No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA188430.11285912416576006No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG187220.11213440124350474No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG184060.11024173642174705No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG181010.10841495550201258No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC178770.10707331967899447No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA178480.10689962575547872No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)