FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17806824
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1734850.9742613281290363Illumina Single End PCR Primer 1 (100% over 50bp)
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC212480.11932504078211814No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG207570.11656767091088224No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA202370.11364744212668132No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG196510.11035656891987027No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA182100.10226416569288269No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA181410.10187667379651756No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)