FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17144338
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG1579310.9211845916710228No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA1500140.8750060807247267No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1405310.8196933588220203No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT848880.4951372283957537No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT745890.4350649176421977No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG744040.4339858441894928No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG698680.4075281296950632No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC607700.3544610471398779No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG583680.3404505907431363No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA531400.30995655825264296No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG517070.30159811361628547No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG483230.28185981867599674No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT481810.28103155689067727No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG473910.2764236216061536Illumina Single End PCR Primer 1 (100% over 50bp)
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT469930.2741021554754695No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC440280.25680781608482056No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT416690.24304817135546442No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA407140.23747781920771746No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT397240.23170331802837765No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG385380.22478558227211806No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA380710.22206165090772242No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT380520.22195082714771489No Hit
ACTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACAC371520.21670128062104232No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC369810.21570386678097458No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT366680.21387819115558734No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA347720.20281914647273053No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT341860.1994011084009193No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC332320.19383658908264642No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC327290.19090267585718387No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT316180.18442240231148033No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG307770.17951699272377852No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA302640.17652475120357522No Hit
CCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATAC289830.16905289664727796No Hit
TAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATAT286090.1668714184239718No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC278470.1624268023647224No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC276020.16099775914357264No Hit
TGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGC273910.15976703212454163No Hit
CAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCAC259080.1511169460144801No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA255180.1488421425195887No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC250090.14587323231728166No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC245810.14337678130237516No Hit
GCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCTC241440.14082783482220193No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC236840.13814473326412485No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG234680.13688484209772345No Hit
CCCAACTAAAAATATTAAACACAAACTACCACCTACCTCCCTCACCAAAG226750.13225940832477756No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA221440.12916217587404075No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG214280.12498586997059903No Hit
CGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATT207750.12117703232402441No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT206380.12037793468607537No Hit
CCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAA199070.11611413634052246No Hit
CTCCAACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAAT199050.1161024706815743No Hit
TGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCT197830.11539086548573646No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC189440.11049712155698284No Hit
CCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAG188110.10972135523693012No Hit
AAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTAT177090.1032935771564933No Hit
ACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATC175280.10223783502168471No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG174730.10191702940061027No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT173860.10140957323636526No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)