FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16800893
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTAC1029830.6129614657982763No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA661840.39393144162039484No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC599790.35699888095233984No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT511590.30450167142901274No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG495760.29507955321184415No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG489770.29151426653333246No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAA449750.2676941041169657No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA387410.23058893357632837No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTA383070.22800573755216466No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT346490.20623308534849905No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT345880.2058700094096189No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT342820.2040486776506463No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACCC315490.18778168517590105No Hit
CTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAG314180.1870019647169945No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG306900.1826688617087199No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT286370.1704492731428026No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC286360.1704433210782308No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT262540.15626550326818936No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACC245600.14618270588355034No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG222750.1325822383369741No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG220400.13118350316259975No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG219930.13090375612772487No Hit
CCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA218040.1297788159236536No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA213340.12698134557490487No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC209300.12457671148789531No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC204890.12195185101172895No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT197190.11736876129143849No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT194990.11605930708564122No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT194380.11569623114676107No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG194010.11547600475760426No Hit
GCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCTC186460.11098219600589089No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT186330.1109048191664574No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA175120.10423255478146311No Hit
CCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAAT174760.1040182804568781No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC171360.10199457850246411No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)