FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20784843
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1065540.512652416955952No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG723770.3482200948065858No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC558040.26848410642312764No Hit
GGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGC545990.26268661254742215No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG438720.21107688905805064No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT431850.2077715958691629No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA427660.20575570380781802No Hit
CAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGCTGC413360.19887569032876504No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC322310.15506973037996968No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC305240.14685701498924No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC296820.14280598607360181No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC251900.12119408359254867No Hit
GACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGG243410.11710937628925079No Hit
CCCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTAC240220.11557460405161588No Hit
GGCAAGACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGC234390.1127696754793866No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT225210.1083529954977288Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)