FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17277101
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1079810.6249948993178891No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT853990.4942901010997158No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC746670.43217319850129954No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA675990.3912635574683508No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT643820.3726435355098057No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG609390.35271542372762654No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG579310.33530509545553966No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG565410.3272597642393825No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA510050.29521735156841417No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC441300.2554247960928167No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG417240.2414988486783749No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT388950.22512457385067092No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT363370.21031884920971405No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG360040.20839144252267783No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG348070.201463196863872No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG344180.19921166172496185No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT334240.1934583817041991No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT321420.18603815535951315No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT309530.17915621376526075No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG309120.17891890543442446No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA308450.17853110889378954No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT289820.16774804986091127No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA278650.16128284484763966No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC269370.15591157335944267No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA268090.1551707083265879No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG263040.1522477642516531No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG258580.1496663126527998No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC245980.14237342248563575No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT242430.14031867962107764No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA232510.13457697561645324No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC229560.1328695132360458No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG225200.13034594171788427No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC225000.1302301815565007No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC215930.12498045823775643No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACC214050.123892312720751No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT208570.12072048429884158No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT207610.12016483552420049No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC206960.11978861499970395No Hit
CGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTG204400.11830688493399442No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG202180.11702194714263695No Hit
GCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAAC196090.11349705022850767No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT188480.10909237608786335No Hit
CACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATG184160.10659195660197854No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC181650.1051391665766149No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC179710.10401629301119442No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT178220.1031538798088869No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG176420.1021120383564349No Hit
CGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGG173270.10028881581464391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)