FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15993685
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA585830.36628831942107154No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA521280.32592863996008425No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA408060.2551381998582566No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG350740.2192990545956107No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG318180.19894101953364718No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC275010.17194911616678707No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG179150.11201296011519547No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC171250.10707351057620555No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC164620.10292812444411654TruSeq Adapter, Index 3 (100% over 50bp)
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG162150.10138376490471082No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)